Raman-spectroscopy-based noninvasive microanalysis of native lignin structure.
نویسندگان
چکیده
A new robust, noninvasive, Raman microspectroscopic method is introduced to analyze the structure of native lignin. Lignin spectra of poplar, Arabidopsis, and Miscanthus were recovered and structural differences were unambiguously detected. Compositional analysis of 4-coumarate-CoA ligase suppressed transgenic poplar showed that the syringyl-to-guaiacyl ratio decreased by 35% upon the mutation. A cell-specific compositional analysis of basal stems of Arabidopsis showed similar distributions of S and G monolignols in xylary fiber cells and interfascicular cells.
منابع مشابه
Mineralogy, Raman spectroscopy and lattice parameter characterization of biotite with hexagonal structure from Borujerd hornfels and migmatites
Biotite is a common rock-forming mineral and accepts a variety of elements in its structure. The valuable information of crystallization conditions of the biotite mineral obtain on natural biotite composition. The biotite mineral is one of the main mineral phases of pelitic rocks which are formed in a wide range of geological conditions. In this research, microscopic mineralogy, Raman spectrosc...
متن کاملADVANCING RAMAN SPECTROSCOPY FOR LlGNlN APPLICATIONS
Over last 25 years, lignin applications of Raman spectroscopy have come a long way. This article provides a brief overview of the advances in the field of lignin. The paper heavily draws on author’s own research although where appropriate examples are included from published work of other researchers. It is emphasized that the developments in Raman instrumentation along with applications of var...
متن کاملLignin Quantitation by Ft-raman Spectroscopy
Because of the structural complexity of biomass, quantitation of lignin in a variety of wood and plant cell walls is difficult. Consequently, to measure lignin in different plant species, a number of different methods exist. The methods can give different values for the same material samples and such differences result from the nature of the biomass. In the past, although there have been many a...
متن کاملNoninvasive Scanning Raman Spectroscopy and Tomography for Graphene Membrane Characterization
Graphene has extraordinary mechanical and electronic properties, making it a promising material for membrane-based nanoelectromechanical systems (NEMS). Here, chemical-vapor-deposited graphene is transferred onto target substrates to suspend it over cavities and trenches for pressure-sensor applications. The development of such devices requires suitable metrology methods, i.e., large-scale char...
متن کاملState of the Art Microanalysis Using Raman Microscopy
Introduction Raman spectroscopy is one of the most common techniques for materials identification and characterization. Based on inelastic scattering of a monochromatic excitation source, Raman measures vibrational, rotational and other low-frequency modes in a system. It’s not only characteristic for functional groups, but also sensitive to backbone and crystalline structures. Therefore, Raman...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Analytical and bioanalytical chemistry
دوره 402 2 شماره
صفحات -
تاریخ انتشار 2012